Determination of DNA Concentration and Purity by Ultraviolet Spectrophotometry
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چکیده
Typically, the first thing one wants to know about a DNA preparation is its concentration and purity. Both can be determined by measuring the absorption of ultraviolet light. DNA absorbs UV more or less strongly depending upon the wavelength. We will be using a NanoDrop spectrophotometer, which takes measurements at wavelengths of 260 and 280 nm, and in addition determines an absorption spectrum from 220 – 350 nm. The graph below shows that maximum absorbance occurs over a broad peak around 260 nm. At 280nm DNA only absorbs about half as much UV.
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